ERK activity sensors
Head of the research group: ATTILA REMÉNYI
Research concept
Cell proliferation may be pathologically promoted by mutations in cell growth promiting promoting kinases. Increased phosphorylation of extracellular signal regulated kinase (ERK) is a hallmark of cancer cells and thus these cells have increased ERK activity in the resting state or they respond to lower amounts of growth hormones. Our goal is to develop ERK activity sensors so that to detect the activity of this pivotal enzyme in cell growth regulation.
Human resources
- Attila Reményi, PhD, Scientific Advisor
- Ádám Levente Póti, research fellow
Labiratories, rooms
Infrastructure for carrying out general molecular biology experiments, protein production and expression plasmid design. Protein-protein interaction measurements in vitro (ITC, SPR and labelling based techniques) and in cell-based assays using protein fragment complementation based methodology. Chemical synthesis of peptides and purification (HPLC/MS).
Equipments
- Hamiltan Starlet pipetting station integrated with plate-readers to carry out high-throughput biochemical assays.
- BioTek Cytation 3 plate-reader with cellular imaging capacity